|Center for Neurogenomics and Cognitive Research – VU Amsterdam|
|CNCR-VU University Amsterdam|
|de Boelelaan 1087|
R v Kesteren
H de Wit
|Live cell imaging – vesicle transport and capture
2PSLM – calcium imaging
TIRF – vesicle dynamics
Spinning disk – cellular dynamics
THG – label free imaging
EM – CLEM
In vivo photostimulation
|Our mission is to focus on biological mechanisms underlying common mental health and neurological brain diseases, applying the latest technology and thereby providing new perspectives on therapy. Major research lines are: – The molecular mechanisms that transport and recruit secretory vesicles to the plasma membrane, their calcium dependent release and the effect of secreted cargo on synaptic plasticity.
– To study how synaptic transmission and plasticity of synaptic contacts changes during key periods of development or via pharmacological manipulation in the hippocampus and cortex.
– Molecular mechanisms underlying synapse formation and synaptic plasticity
|– de Wit, J, Toonen RF, and Verhage M. Matrix-dependent local retention of secretory vesicle cargo in cortical neurons. (2009) J Neurosci 29, 23-37.
– Meredith RM*, Holmgren CD*, Weidum M, Burnashev N, Mansvelder HD. (2007) Increased threshold for spike-timing-dependent plasticity is caused by unreliable calcium signaling in mice lacking fragile X gene FMR1. Neuron. 54:627-38.
– Toonen RF*, Kochubey O*, de Wit H*, Gulyas-Kovacs A*, Konijnenburg B, Sorensen JB, Klingauf J, Verhage M. Dissecting docking and tethering of secretory vesicles at the target membrane. (2006) EMBO J. 25(16):3725-37.
– Witte S, Negrean A, Lodder JC, de Kock CP, Testa-Silva G, Mansvelder HD*, Groot ML* (2011) Label-free live brain imaging and targeted patching with third-harmonic generation microscopy. Proc Natl Acad Sci U S A. 108(15):5970-5.
|Zeiss CSLM 510 Meta
Zeiss axiovert 200M TIRF equipped with UGA-40 photostimulation system
Zeiss axiovert 200 TIRF
Olympus IX-81 equipped with UGA-40 photostimulation system
Olympus IX-81 spinning disk
LaVision Trim scope 2PSLM with OPO and Ti:Sa laser
Custom build in vivo 2PSLM
Opera & Cellomics automated confocal cellular imaging and screening
|– VU University Master course Advanced Live Cell Imaging
– ONWAR PhD course Live Cell Imaging