| MicroSpectroscopy Centre | |
| JanWillem.Borst@wur.nl This e-mail address is protected from spam bots, you must enable JavaScript in your web browser to view it |
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| J.W. Borst | |
| Wageningen University | |
| Dreijenlaan 3 | |
| 6703 HA | |
| Wageningen |
| J.W. BorstA.N. BaderJ. Hohlbein
H. van Amerongen D. Weijers |
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| Fluorescence Lifetime Imaging Microscopy (FLIM)Fluorescence Correlation Spectroscopy (FCS)Förster Resonance Energy Transfer (FRET)
Widefield Microscopy Confocal Laser Scanning Microscopy (CLSM) + FRET + (Spectral) FLIM Multi-Photon Microscopy (MPM) + FRET + FLIM Single Molecule Microscopy (SMM) +FRET + super-resolution microscopy Time Resolved Fluorescence (TRF) + Anisotropy , Steady state fluorescence spectroscopy/anisotropy Circular Dichroism (CD) Expertise: plant tissue imaging/spectroscopy, food microscopy, advanced FLIM analysis (global lifetime fitting and phasor approach) |
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| Research within the MSC is largely centered around the plant sciences and focuses on cellular organization, signal transduction, development, plant pathogens and plant symbionts, but also more applied questions on plant morphology are addressed that originating from the market. Functional studies are conducted on protein folding and stability, biomolecular networks and light stress in photosynthesis. Other topics include biosensors, biobased materials and food properties. | |
| Monitoring photosynthesis in individual cells of Synechocystis sp. PCC6803 on a picosecond timescale. Krumova, S.B., Laptenok, S. P., Borst, J. W., Ughy, B., Gombos, Z., Ajlani, G., van Amerongen, H. Biophys. J. 99: 2006-2015 (2010).Global analysis of Förster resonance energy transfer in live cells measured by fluorescence lifetime imaging microscopy exploiting the rise time of acceptor fluorescence. Laptenok SP, Borst JW, Mullen KM, van Stokkum IH, Visser AJ, van Amerongen H. Phys Chem Chem Phys. 21: 7593-7602 (2010).Nolles A, Westphal AH, de Hoop JA, Fokkink RG, Kleijn JM, van Berkel WJ, Borst JW. Encapsulation of GFP in Complex Coacervate Core Micelles. Biomacromolecules. 2015 May 11;16(5):1542-1549.
Lindhoud S, Westphal AH, van Mierlo CP, Visser AJ, Borst JW. Rise-time of FRET-acceptor fluorescence tracks protein folding. Int J Mol Sci. 2014 Dec 19;15(12):23836-23850 Bücherl CA, Bader A, Westphal AH, Laptenok SP, Borst JW. FRET-FLIM applications in plant systems. Protoplasma. 2014 Mar;251(2):383-394 Bücherl CA, van Esse GW, Kruis A, Luchtenberg J, Westphal AH, Aker J, van Hoek A, Albrecht C, Borst JW, de Vries SC. Visualization of BRI1 and BAK1(SERK3) membrane receptor heterooligomers during brassinosteroid signaling. Plant Physiol. 2013 Aug;162(4):1911-1925 Rademacher EH, Lokerse AS, Schlereth A, Llavata-Peris CI, Bayer M, Kientz M, Freire Rios A, Borst JW, Lukowitz W, Jürgens G, Weijers D. Different auxin response machineries control distinct cell fates in the early plant embryo. Dev Cell. 2012 Jan 17;22(1):211-222. |
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| Zeiss LSM 510/Confocor2Zeiss Confocor1
Leica DRM6000 DIC -fluorescence microscope BioRad Radiance 2100MP Two-photon NDD-FLIM Leica SP5-X (internal HyD detectors) Leica SP5-X including SMD unit for spectral FLIM and FCS Home built Single molecule Total Internal Reflection Fluorescence microscope |
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| www.mscwu.wur.nl | |
| FRET spectroscopyFRET-FLIM in plants and protoplasts
FCS in vitro and in vivo plant tissue imaging Single molecule localisation microscopy |
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| FEBS advanced practical and lecture courses are organized biannually. Courses on Microspectroscopy for PhD students within different EU networksWageningen University courses:Advanced Methods in Biochemical ResearchBiophysical ImagingCell Biology and Advanced Imaging Technologies
Biophotonics and Photosynthesis |
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| Yes |