Department of Molecular Cell Biology

Department of Molecular Cell Biology<br />
This e-mail address is protected from spam bots, you must enable JavaScript in your web browser to view it<br />
H.J. Tanke
Leiden University Medical Center
Einthovenweg 20
2300 RC
Prof.Dr H.J. Tanke,Prof.Dr A.J. Koster,

Dr J. Vrolijk,

Dr R.W. Dirks,

Dr C.R. Jost

Dr. F.G.A. Faas,

Dr. R. Koning,

Dr. J. Wiegant,

Ing. W.C.R. Sloos

Ing. A. Boonzaier-van der Laan

Aim of this research program is to develop and/or adapt microscopic imaging methods, instrumentation and fluorescent labeling technologies to study the molecular composition of tissues, cells and subcellular compartments. The programme involves both light and electron microscopy and supports projects within the realm of both cell- and structural biology.Examples of developments are two-photon or confocal laser scanning microscopy to study molecular dynamics in living cells using FRET/FRAP techniques, time-lapse fluorescence imaging of living cells, two-photon intravital microscopy of small laboratory animals, digital fluorescence workstations to perform 48 colour FISH karyotyping in case of malignancies and pre/postnatal abnormalities. For high-resolution (nm-scale) ultrastructural studies, the core electron microscopic imaging tools include (cryo) transmission electron microscopy (TEM) with energy-filtering, (cryo) scanning electron microscopy (SEM) and electron tomography (3D imaging of cellular organelles and macromolecules). Ongoing technical developments aim at improving specimen preparation and labeling techniques and at advancing further automation of methodologies. A key development is aimed at correlative light-electron microscopy, allowing the combination of high-resolution imaging with electron microscopy with live-cell dynamic imaging with fluorescence light microscopy. In addition the group develops new fluorescent reporter molecules (such as luminescent crystals that are used in point-of care-tests using lab-on-a-chip devices), cytochemical staining procedures and dedicated software for cell and structural analysis. Applications of microscopic imaging and technology range from fundamental cell biological studies, imaging the cellular ultrastructure and/or dynamics with electron microscopy or live cell microscopy, to diagnostic applications of multicolour FISH and reported technology in case of oncology, genetics and infectious diseases
Bárcena M, Oostergetel GT, Bartelink W, Faas FG, Verkleij A, Rottier PJ, Koster AJ, Bosch BJ. Cryo-electron tomography of mouse hepatitis virus: Insights into the structure of the coronavirion. Proc. Natl. Acad. Sci. USA. 2009 Jan 13;106(2):582-7. Epub 2009 Jan 5; (IF: 9.8)Szuhai K, Ijszenga M, de Jong D, Karseladze A, Tanke HJ, Hogendoorn PC. The NFATc2 gene is involved in a novel coned translocation in a Ewing sarcoma variant that couples its function in immunology to oncology. Clin Cancer Res. 2009 Apr 1;15(7):2259-68. Epub 2009 Mar 24. (IF: 7.3)

Bárcena M, Koster AJ Electron tomography in life science. Semin. Cell Dev. Biol. 2009 Oct;20(8):920-30. (IF: 5.9)

Brouwer AK, Schimmel J, Wiegant JC, Vertegaal AC, Tanke HJ, Dirks RW. Telomeric DNA mediates de novo PML body formation. Mol. Biol. Cell. 2009 Nov;20(22):4804-15. Epub 2009 Sep 30 (IF: 5.9)

Asp L, Kartberg F, Fernandez-Rodriguez J, Smedh M, Elsner M, Laporte F, Bárcena M, Jansen KA, Valentijn JA, Koster AJ, Bergeron JJ, Nilsson T. Early stages of Golgi vesicle and tubule formation require diacylglycerol. Mol. Biol. Cell. 2009 Feb;20(3):780-90. (IF: 5.9)

Janssen BJ, Gomes L, Koning RI, Svergun DI, Koster AJ, Fritzinger DC, Vogel, CW, Gros P. Insights into complement convertase formation based on the structure of the factor B-cobra venom factor complex. EMBO J. 2009 28(16)2469-78. (IF: 10.1)

Knijnenburg J, Oberstein SA, Frei K, Lucas T, Gijsbers AC, Ruivenkamp CA, Tanke HJ, Szuhai K. A homozygous deletion of a normal variation locus in a patient with hearing loss from non-consanguineous patients. J. Med. Genet. 2009 Jun;46(6):412-7. Epub 2009 Feb 25 (IF 7.0)

van Gemert AM, van der Laan AM, Pilgram GS, Fradkin LG, Noordermeer JN, Tanke HJ, Jost CR. In vivo monitoring of mRNA movement in Drosophila body wall muscle cells reveals the presence of myofiber domains. PLoS One. 2009 Aug 17;4(8):e6663 (IF: 4.4)

Valentijn KM, van Driel LF, Mourik MJ, Hendriks GJ, Arends TJ, Koster AJ, Valentijn JA.Multigranular exocytosis of Weibel-Palade bodies in vascular endothelial cells. Blood. 2010 Sep 9;116(10):1807-16 (IF: 10.6)

EMBO Course on cryo-electron tomography
EMBO Course on cryo-electron tomography