Erasmus Optical Imaging Centre (OIC)

Erasmus Optical Imaging Centre (OIC)
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Dr. Ir. Gert-Jan Kremers
Erasmus MC
Wytemanweg 80
3015 CN
Prof. Dr. A.B. Houtsmuller
Dr. W.A. van Cappellen
Dr. Ir. G.J. Kremers
Dr. J. Slotman
Ir. M. Paul
A.L. Nigg
H. de Gruiter
B. Geverts
Confocal microscopy
Live cell imaging
Intra-vital imaging
Spinning disc microscopy
TIRF microscopy
Multi-photon microscopy
Structured illumination microscopy (SIM)
Photoactivation localization Microscopy (PALM)
direct Stochastical optical reconstruction microscopy (dSTORM)
4Pi microscopy
Fluorescence redistribution after photobleaching (FRAP)
Local DNA damage induction (MP, UV)
Single molecule imaging and tracking in living cells and fixed material
Förster Resonance energy transfer (FRET)
Fluorescence correlation spectroscopy (FCS)
Combinations of these techniques
Laser capture microdissection
Image and data analysis
FRAP Monte Carlo simulations
Virtual cell simulations
Automated timelapse image analysis (ImageJ)
Functional imaging of biomedical processes
Quantitative analysis of FRAP data
DNA repair
Gene transcription
Nuclear receptors
Chromatin structure and function
Stem cell development
Tumor manipulation and targeting
Agarwal S, van Cappellen WA, Guénolé A, Eppink B, Linsen SE, Meijering E, Houtsmuller AB, Kanaar R, Essers J. ATP-dependent and independent functions of Rad54 in genome maintenance. J Cell Biol. 2011 192:735-50Boisset JC, van Cappellen W, Andrieu-Soler C, Galjart N, Dzierzak E, Robin C. In vivo imaging of haematopoietic cells emerging from the mouse aortic endothelium. Nature. 2010 464:116-20Dzyubachyk O, Essers J, van Cappellen WA, Baldeyron C, Inagaki A, Niessen WJ, Meijering E. Automated analysis of time-lapse fluorescence microscopy images: from live cell images to intracellular foci. Bioinformatics. 2010 26:2424-30.van Royen ME, Cunha SM, Brink MC, Mattern KA, Nigg AL, Dubbink HJ, Verschure PJ, Trapman J, Houtsmuller AB. Compartmentalization of androgen receptor protein-protein interactions in living cells. J Cell Biol. 2007 177: 63-72Houtsmuller AB, Rademakers S, Nigg AL, Hoogstraten D, Hoeijmakers JH, Vermeulen W. Action of DNA repair endonuclease ERCC1/XPF in living cells. Science. 1999 284:958-61.
Zeiss LSM510 NLO Confocor II
Zeiss LSM510 Meta with 266nm UV laser
Zeiss LSM510 Meta intravital
Zeiss LSM510 Meta
Zeiss LSM700 upright
Zeiss Elyra PS1 PALM SIM + LSM780 + GaAsP
Zeiss NIR fluorescence widefield microscope
Zeiss PALM micro dissection
Leica SP5 Multi photon + 266nm UV laser + APD
Leica SP5 APD
Leica SP5 HyD
Leica SP5 upright intra-vital + Multiphoton + OPO + NDD
Leica 4Pi
Nikon / Roper TIRF + FRAP + single molecule detection
Nikon / Roper Spinning disc + FRAP
Nikon Ti-Eclipse fluorescence widefield microscope
CytoMate incubator microscope
All OIC equipment is also available for users from outside the Erasmus MC.
International Ph.D./postdoc courses:
Functional Imaging and Super Resolution (annually in October):
One week intensive advanced microscopy course combining theory and hands-on sessions.

Quantitative Fluorescence Microscopy at MDIBL, Maine, USA (annually in May):
One week intensive microscopy course combining theory and hands-on sessions.

M.Sc. course:
Optical Imaging and live cell microscopy (annually in October):
Organized in collaboration with the Infection & Immunity, Neuroscience and Molecular Medicine research master schools at Erasmus MC.

B.Sc. courses:
The OIC participates in the curriculum of the Nanobiology bachelor program of Erasmus MC/Technical University Delft.

Other courses:
ImageJ Image analysis course (annually in January): Two days interactive course covering the basic principles of image processing using ImageJ.

Confocal introduction courses (monthly): One day course required to learn the basics of operating a confocal microsope.

Housing for external users available?: No