MicroSpectroscopy Centre

MicroSpectroscopy Centre
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J.W. Borst
Wageningen University
Dreijenlaan 3
6703 HA
J.W. BorstA.N. BaderJ. Hohlbein

H. van Amerongen

D. Weijers

Fluorescence Lifetime Imaging Microscopy (FLIM)Fluorescence Correlation Spectroscopy (FCS)Förster Resonance Energy Transfer (FRET)

Widefield Microscopy

Confocal Laser Scanning Microscopy (CLSM) + FRET + (Spectral) FLIM

Multi-Photon Microscopy (MPM) + FRET + FLIM

Single Molecule Microscopy (SMM) +FRET + super-resolution microscopy

Time Resolved Fluorescence (TRF) + Anisotropy ,

Steady state fluorescence spectroscopy/anisotropy

Circular Dichroism (CD)

Expertise: plant tissue imaging/spectroscopy, food microscopy, advanced FLIM analysis (global lifetime fitting and phasor approach)

Research within the MSC is largely centered around the plant sciences and focuses on cellular organization, signal transduction, development, plant pathogens and plant symbionts, but also more applied questions on plant morphology are addressed that originating from the market. Functional studies are conducted on protein folding and stability, biomolecular networks and light stress in photosynthesis. Other topics include biosensors, biobased materials and food properties.
Monitoring photosynthesis in individual cells of Synechocystis sp. PCC6803 on a picosecond timescale. Krumova, S.B., Laptenok, S. P., Borst, J. W., Ughy, B., Gombos, Z., Ajlani, G., van Amerongen, H. Biophys. J. 99: 2006-2015 (2010).Global analysis of Förster resonance energy transfer in live cells measured by fluorescence lifetime imaging microscopy exploiting the rise time of acceptor fluorescence. Laptenok SP, Borst JW, Mullen KM, van Stokkum IH, Visser AJ, van Amerongen H. Phys Chem Chem Phys. 21: 7593-7602 (2010).Nolles A, Westphal AH, de Hoop JA, Fokkink RG, Kleijn JM, van Berkel WJ, Borst JW. Encapsulation of GFP in Complex Coacervate Core Micelles. Biomacromolecules. 2015 May 11;16(5):1542-1549.

Lindhoud S, Westphal AH, van Mierlo CP, Visser AJ, Borst JW. Rise-time of FRET-acceptor fluorescence tracks protein folding. Int J Mol Sci. 2014 Dec 19;15(12):23836-23850

Bücherl CA, Bader A, Westphal AH, Laptenok SP, Borst JW. FRET-FLIM applications in plant systems. Protoplasma. 2014 Mar;251(2):383-394

Bücherl CA, van Esse GW, Kruis A, Luchtenberg J, Westphal AH, Aker J, van Hoek A, Albrecht C, Borst JW, de Vries SC. Visualization of BRI1 and BAK1(SERK3) membrane receptor heterooligomers during brassinosteroid signaling. Plant Physiol. 2013 Aug;162(4):1911-1925

Rademacher EH, Lokerse AS, Schlereth A, Llavata-Peris CI, Bayer M, Kientz M, Freire Rios A, Borst JW, Lukowitz W, Jürgens G, Weijers D. Different auxin response machineries control distinct cell fates in the early plant embryo. Dev Cell. 2012 Jan 17;22(1):211-222.

Zeiss LSM 510/Confocor2Zeiss Confocor1

Leica DRM6000 DIC -fluorescence microscope

BioRad Radiance 2100MP Two-photon NDD-FLIM

Leica SP5-X (internal HyD detectors)

Leica SP5-X including SMD unit for spectral FLIM and FCS

Home built Single molecule Total Internal Reflection Fluorescence microscope

FRET spectroscopyFRET-FLIM in plants and protoplasts

FCS in vitro and in vivo

plant tissue imaging

Single molecule localisation microscopy

FEBS advanced practical and lecture courses are organized biannually. Courses on Microspectroscopy for PhD students within different EU networksWageningen University courses:Advanced Methods in Biochemical ResearchBiophysical ImagingCell Biology and Advanced Imaging Technologies

Biophotonics and Photosynthesis