UMCG Microscopy and Imaging Center

UMCG Microscopy and Imaging Center
University Medical Center Groningen
Antonius Deusinglaan 1
B.N.G. Giepmans

K.A. Sjollema

CLSM, Live-cell imaging, Intra-vital imaging, Two photon microscopy, Spinning disk CLSM, Large scale imaging/analysis, FRET, FRAP, Correlative LM/TEM, high speed (fluorescence) microscopy, TEM, SEM,STEM, FCS,  3D-reconstruction, deconvolution, wide-field fluorescence.
Diabetes, Probe development, Cell Biology, Neural networks, etc.
Immunolabeling artifacts and the need for live-cell imaging.

Schnell U, Dijk F, Sjollema KA, Giepmans BN.

Nat Methods. 2012 Jan 30;9(2):152-8.Correlated light microscopy and electron microscopy.

Sjollema KA, Schnell U, Kuipers J, Kalicharan R, Giepmans BN.

Methods Cell Biol. 2012;111:157-73.

Differential Effects of TNF (TNFSF2) and IFN-γ on Intestinal Epithelial Cell Morphogenesis and Barrier Function in Three-Dimensional Culture.

Juuti-Uusitalo K, Klunder LJ, Sjollema KA, Mackovicova K, Ohgaki R, Hoekstra D, Dekker J, van Ijzendoorn SC.

PLoS One. 2011;6(8):e22967

Rapid and efficient cancer cell killing mediated by high-affinity death receptor homotrimerizing TRAIL variants.

Reis CR, van der Sloot AM, Natoni A, Szegezdi E, Setroikromo R, Meijer M, Sjollema K, Stricher F, Cool RH, Samali A, Serrano L, Quax WJ.

Cell Death Dis. 2010 Oct 21;1:e83.

IC. elegans model identifies genetic modifiers of alpha-synuclein inclusion formation during aging.

van Ham TJ, Thijssen KL, Breitling R, Hofstra RM, Plasterk RH, Nollen EA.

PLoS Genet. 2008 Mar 21;4(3):e1000027.

Expression, transport, and axonal sorting of neuronal CCL21 in large dense-core vesicles.

de Jong EK, Vinet J, Stanulovic VS, Meijer M, Wesseling E, Sjollema K, Boddeke HW, Biber K.

FASEB J. 2008 Dec;22(12):4136-45. Epub 2008 Aug 12.


Solamere Nipkow Disk CLSM with I-CCd. 37dgr cabinet

Till iMIC fast fluorescence microscope with EM-CCD

Tissuegnostics/Zeiss TissueFaxs large scale/high throughput microscope

Leica DM 4000B fluorescence mic.

Olympus BX50 research microscope

Zeiss LSM 780 NLO/OPO 37 dgr cabinet (end this year)

FEI CM100 Transmission EM

JEOL 6301F Scanning EM
Individual training for users

Open for users from UMCG and RUG and other institutions.

Annual PhD course “cellular imaging”