Dept. of Molecular Cell Biology and Immunology

Dept. of Molecular Cell Biology and Immunology
E. Hendrikx
VUmedisch centrum
van der Boechorststraat 7
Prof. Dr. C.D. Dijkstra

Prof. Dr. R. Beelen

Prof. Dr. R. Mebius

Prof. Dr. H.E. de Vries

Dr. E. van Egmond

High resolution microscopy in flow

Live- cell imaging,




Image analysis

The research program of the department of Molecular Cell Biology and Immunology is coherent with a focus is on immune development and regulation, host-pathogen interaction and chronic inflammation. Emphasis is on questions concerning cellular interactions, cell surface molecules involved in these interactions and signalling pathways within the cells that regulate cell function and differentiation.

Various approaches are being applied to answer the research questions, varying from biochemical and molecular methods, cell biological techniques and animal models, to translational approaches, in order to apply the generated knowledge for clinical (diagnostic or therapeutic) application. In particular basic questions relevant for cancer, inflammatory bowel diseases, kidney diseases and CAPD, multiple sclerosis and vascular diseases are being addressed.

Research groups:

Inflammation and wound healing

Glycobiology and immune regulation


Tumor immunology

Vascular inflammation

Dendritic cell immunobiology

Immune regulation

Functional development of the immune system

Blood Brain Barrier

– Reijerkerk A, Lakeman K, Drexhage J, van het Hof B, van Wijck Y, van der Pol SMA, Kooij G, Geerts D, de Vries HE. 2011. Brain endothelial barrier passage by monocytes is controlled by the endothelin system, J. Neurochem. 10.1111/j.1471-4159.2011.07393.x

– van Doorn R, van Horssen J, Verzijl D, Witte M, Ronken E, van het Hof B, Lakeman K, Dijkstra CD, van der Valk P, Reijerkerk A, Alewijnse AE, Peters SLM, de Vries HE. 2010. Sphingosine 1-phosphate receptor 1 and 3 are upregulated in multiple sclerosis lesions. Glia 58:1465-1476

– Schreibelt G, Musters RJ, Reijerkerk A, de Groot LR, van der Pol SM, Hendrikx E, Döpp ED, Dijktsra CD, Drukarch B, de Vries HE. 2006. Lipoic acid affects cellular migration into the central nervous system and stabilizes blood-brain barrier integrity. J. Immunol. 177(4):2630-7

– Reijerkerk A, Kooij G, van der Pol SM, Khazen S, Dijkstra CD, de Vries HE. 2006. Diapedesis of monocytes is associated with MMP-mediated occludin disappearance in brain endothelial cells. FASEB J 20(14):2550-2

– van de Pavert, SA; Olivier, BJ; Goverse, G; et al.2009. Chemokine CXCL13 is essential for lymph node initiation and is induced by retinoic acid and neuronal stimulation. NATURE IMMUNOLOGY Volume: 10 Issue: 11 Pages: 1193-U78

– von den Hof, MF; van de Pavert, SA; Dillard,ME; et al. 2009. Lymph sacs are not required for the initiation of lymph node formation. DEVELOPMENT Volume: 136 Issue: 1 Pages: 29-34

– van de Pavert, SA; Meuleman, L; Malysheva, A; et al. 2007. A single amino acid substitution (Cys249Trp) in Crb1 causes retinal degeneration and deregulates expression of pituitary tumor transforming gene Pttg1. JOURNAL OF NEUROSCIENCE Volume: 27 Issue: 3 Pages: 564-573

– Seeliger, MW; Beck, SC; Pereyra- Munoz, N; et al. 2005. In vivo confocal imaging of the retina in animal models using scanning laser ophthalmoscopy. VISION RESEARCH Volume: 45 Issue: 28 Pages: 3512-3519

– Fisher, MD; Huber, G; Beck, SC; et al. 2009. Noninvasive, In Vivo Assessment of Mouse Retinal Structure Using Optical Coherence Tomography. PLOS ONE Volume: 4 Issue: 10

– High resolution microscopy in flow: Amnis ImageStream

– Confocal microscope: Leica SP2 AOBS with inverted microscope (Leica DM IRE2) and POC cell cultivation system.

– Two photon microscope: Lavision TrimScope II with Coherent TI:Sapphire ultra II laser, upright microscope Olympus BX51WI and intravital microscopy stage. The machine features 64-beam technology for fast imaging and a EM-CCD camera (Hamamatsu C9100).

– Olympus IX81 inverted microscope with climate chamber and zero drift compensation (ZDC) system.

– Time lapse system Nikon TE 300 microscope with incubator.

– Electron microscope: Philips CM100 Bio

– Leica DM6000B with DFC 350FX and DFC 320 CCD camera;

– Nikon E800 with DMX1200

– Leica DM-IL inverted with DFC420C camera

– Nikon SMZ800 Stereo microscope

– Software (Cell(R/F/IM), LAS and LAS-AF, LCS, Imspector) and image analysis software (AnalySIS, Soft Imaging System GmbH).